Chemiluminescent Imaging, Alphaimager HD-2
Summary
FluorChem® HD2 Imaging
System Sensitive Chemiluminescent Imaging
Offering best-in-class resolution, sensitivity and dynamic range, the FluorChem HD2 System is optimized to image chemiluminescent blots at speeds that surpass film.
- Fast lens technology provides the ability to rapidly detect and quantitate chemiluminescent westerns without the need for film and a scanner
- Protocol driven analysis reducing imaging time by remembering the settings for imaging your assay
- Advanced AlphaView software provides the tools for analyzing your data, and provides the ability to save your analysis protocols for your assay
- Faster, more sensitive than film and quantifiable.
- Applications: Chemiluminescent Westerns, 96-Well Plates, 2-D Gels, Colony Counting, Protein Gels
The Digital Darkroom
The FluorChem HD2 System delivers higher sensitivity and a broader dynamic range than film can provide at similar exposure times. Captured images are saved digitally, making them easy to import into presentations and reports.
When low level sample detection is required, FluorChem HD2 offers the highest sensitivity without sacrificing speed for chemiluminescent applications.
FluorChem Systems use large aperture fixed lenses to increase the speed of light collection. This reduces acquisition times and provides image quality that is far superior to typical zoom lenses.

Slot blot of antibody coupled to HRP spotted on PVDF membrane. ChemiGlow® Chemiluminescent Substrate was used.

Stronger Signal in Less Time.

Equivalent Signal in the Same Time.
Features And Benefits
- Motorized optics and Automatic Image Capture Software provide ease of use when imaging
- Multiple lens options allow systems to be customized for specific applications
- Five-position filter wheel provides UV fluorescent imaging versatility
- System upgrades allow expansion of capabilities as application needs change
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Filters |
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Orange Filter (590 nm50 nm Band Pass) |
Ethidium Bromide and SYPRO Orange |
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Red Filter (620 nm40 nm Band Pass) |
SYPRO Red, SYPRO Ruby, Texas Red (Optional) |
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Green Filter (537 nm35 nm Band Pass) |
Fluorescein, SYBR Gold, and GFP (Optional |
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Blue Filter (460 nm40 nm Band Pass) |
Hoechst Blue and Carbohydrate Gels (Optional) |
Specifications
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Description |
Alphaimager HD-2 |
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Dimensions external HxWxD, mm |
100 x 50 x 38 cm |
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Field of View (Fast Lense) |
15 x 15 cm |
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Field of View (motorized lens) |
26 x 23 cm |
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Capacity |
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Weight, kg |
45kgs |
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Wavelength |
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Scientific grade Camera |
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Pixel size |
7.4 Micron Square |
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A/D Convertor |
16 bit |
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Resolution |
4.19 Megapixels |
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Absolutely Regulated |
-25Degrees for consistent imaging over long exposures and variable ambient. |
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Light Sources |
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UV Transilluminator |
302/365 nm Dual Wavelength UV Transilluminator (42 cm x 31 cm) |
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Epi White Light |
Dual Epi White Light Modules |
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Trans White Light |
Fold Down White Light Table(standard) |
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Epi UV |
254 nm, 365 nm or Dual 254/365 nm (Optional) |
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Cabinet |
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Filter Position |
Automated 5-position |
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Construction |
1pieces light tight casting. Upgradeable to Flourescent FC-Q. |
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Optics |
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Manual |
Manual fixed 50 mm f/0.95 (standard Fast Lense optimized for Chemi) |
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Manual |
Manual 28 mm f/1.8 (option lense for flexibility and larger FOV) |
Ordering Information
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Part No |
Description |
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92-15410-00 |
FluorChem HD2 System (Lens must be ordered separately) includes a 4.19 mP camera with 16-bit a/D, cooled to -25°C absolute and regulated, orange filter, white light table, adjustable tray kit, 365/302 nm dual-wavelength, dual-intensity uv transilluminator, epi white lights, alphaview hD2 Software and two stand alone licenses |
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92-13604-00 |
FluorChem HD2 System with Manual Fixed Lens 50 mm, f/0.95 includes a 4.19 mP camera with 16-bit a/D, cooled to -25°C absolute and regulated, 50 mm, f/0.95 fixed lens, orange filter, white light table, adjustable tray kit, 365/302 nm dual-wavelength, dualintensity uv transilluminator, epi white lights, alphaview hD2 Software and two stand alone licenses |
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60-14329-00 |
FluorChem Xplor Upgrade for FluorChem HD2 extends FluorChem hD2 capability to include large format 1D (24 x 24 cm) and 2D protein gel imaging. includes f/1.8 manual fixed optics, red filter, XplorBright, Xploruv, sample tray, Xplor Software and Xpedition 2D analysis Software |
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92-14155-00 |
FluorChem Q Upgrade for FluorChem HD2 includes multiimage® iii Cabinet with 3 specific epi excitation channels, four emission filters, white light table, sample tray, epi white lights, and alphaview Q Software with two stand alone licenses. |
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91-13591-00 |
Manual Fixed Lens 50 mm, f/0.95 |
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91-14473-00 |
Motorized Fixed Lens 50 mm, f/0.95 |
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91-12908-00 |
Manual Fixed Lens 28 mm, f/1.8 |
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91-13166-00 |
Motorized Fixed Lens 28 mm, f/1.8 |
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10-14794-00 |
Computer Comes pre-installed with alphaview System Software and is configured for optimal imaging system performance. |
