• Dendritic and tumor hybrid immunotherapyHybridoma antibody production
• Nuclear transfer for fertility treatment

• Approximately 10X or more efficient than PEG
• Patented AC waveforms to induce dielectrophoresis
• Unique coaxial electrode for optimal pearl chain formation
• Large chambers up to 1 ml volume
• Fully customizable protocol parameters
• Computer controlled operationLog file automatically saved after every run
• Automatic safety shutdown on arc detection
• Built-in voltage and current monitors for oscilloscope verification

Electrofusion Protocol

There are a number of techniques used to fuse dissimilar cells such as B cells and myeloma cells to produce viable hybrids. Cyto Pulse fusion technology consists of a four-step procedure for cell electrofusion: 1) cell alignment by non-uniform alternating electric field, known as dielectrophoresis, 2) membrane disruption and subsequent fusion of adjoining cells by DC pulse, 3) aided maturation of fused cells by reapplication of the alternating electric field, and 4) further maturation conditions prior to culture. Optimum parameter values are dependent on the cell types being fused.

Coaxial Electrode

• Three volume models of 350 µl, 750 µl, and 1000 µlClear Pyrex optically clear bottom for easy cell visibility by regular or inverted microscopeDurable stainless steel body prevents sample contamination1% electrode gap tolerance ensures high repeatability
• Alcohol clean or autoclave up to 10 times

Cytofusion Medium

Critical to the cell fusion process, Cyto Pulse Cytofusion Medium provides users with a low conductivity solution to prevent the persistent complication of cell death from excessive heat buildup, and is extremely important to wash cells at least twice in order to avoid turbulence during the alignment process. In addition, Cytofusion Medium is formulated to optimally balance divalent cations while tightly controlling osmolarity, pH, sterility, and endotoxin levels for ensuring consistent results from fusion to fusion.
 

System Monitoring

There is no mystery to what's inside the box when all necessary information can be accessed from the outside - Included as standard features are one each of voltage and current monitors ready to attach with an external oscilloscope in order to view real-time pulsing conditions. Using these connections is a true safeguard to know exactly how the applied waveform reflects the programmed waveform, so you never again have to wonder if your protocol ran as intended.

In addition, information relevant to each executed protocol is automatically saved as a log file, including magnified voltage levels of all pulses.
 

Fault Detection

All Cyto Pulse Sciences equipment is designed to withstand extreme user conditions. In the event of a fault, advanced control circuitry immediately detects the source of any problem and proceeds to halt the system to prevent permanent damage. The safety of Cyto Pulse customers is our number one priority.

• Electrode Interlock – improperly attached cuvettes or electrodes will cause the protocol start function to be disabled until correctedExternal Fault – extremely low output load arcs will cause high voltage to be turned off and a fault screen to be displayed
• Internal Fault – damaged internal system components will cause high voltage to be turned off and fault screen to be displayed
   

Specifications

PA-101
12.6" x 11.8" x 3"
(32 cm x 30 cm x 7.5 cm)

PA-101
8.8 pounds (4 kg)

Included in the System

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