Biosera
Summary
Beside the 20 amino acids found in building blocks of proteins, many biologically occurring amino acids serve other functions in the cells. Different organisms vary considerably in their ability to synthesise amino acids and therefore require these amino acids produced by plant or bacteria preformed in the diet. These are called essential amino acids. Man and the Albino rat can make only 10 of the 20 common amino acids. The non-essential amino acids can be synthesised directly by the organism but they are also added to cell culture media to save energy for the cells.
Summary
The overall potential of vitamins not only in the field of research but also for therapeutic benefits of humans was realised at an early stage in cell biology. It was discovered that mammalian cells are not able to synthesise this class of biological compounds and the artificial basic cell culture media containing sugars, salts and amino acids were supplemented with the different isolated vitamins. These formulations were later intensified.
Summary
Cell Culture Antibiotics: The use of Antibiotics has become a widely used tool in the cell culture field. Most of the antibiotics suppress the growth of microorganisms by blocking an anabolic pathway. If the Antibiotic required is not listed, please contact us
Summary
The irrigating buffers and salt solutions are sterile physiological balanced solutions intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. These buffers and solutions are not cell culture media. They are composed of a phosphate buffer system, sodium chloride to adjust the osmolarity and perhaps sugar and metal ions for a short time for nutrition and stabilisation of morphology.
Summary
Biosera culture medium formulations are manufactured following original publications and standards set by the Tissue Culture Association and accepted formulations.
Custom Made Media - In addition to standard media, it also possible to custom make media according to your personal requirements. Let us know the components needed and the quantities that correspond and we will produce this to your requirements. There may be a short delay for this service.
Summary
Dilution in cell culture Media
3g of instant FBS is equivalent to 50ml of liquid FBS
3g pre-packed in tubes for 500ml Media (10% FBS)
Advantages:
- Can be stored at 2-8°C for a max of 3 years
- NO freeze/thaw cycles
- NO need for dry ice shipments
Summary
All the sera is processed in a certified ISO9001 facility and most origins of FBS sold are also EDQM certified.
Foetal Bovine Serum is derived from clotted whole blood, collected from the foetus via cardiac puncture. The blood is immediately centrifuged and the serum frozen for transfer to our processing facilities. On receipt at the processing plant, the serum is thawed, tested for acceptability, pooled and passed through three 100nm (0.1um) sterilising filters. The serum is bottled through an asceptic filling process.
