MP-100 Microporator - pipette tip based electroporator
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Brochures

Microporator Brochure

Technical Notes


MicroPorator gives efficient gene delivery to neuronal cell

A transfection of Antisense Oligodeoxynucleotide (ODN) with Microporator

Posters


Microporation technology, a highly efficient gene delivery method for hard-to-transfect cells

Papers

Chromatin Remodeling Complex Interacts with ADD1/SREBP1c To Mediate Insulin-Dependent Regulation of Gene Expression. Mol. Cell. Biol. 27(2): 438-452 [Pubmed]

Frequent Alteration of XAF1 in Human Colorectal Cancers: Implication forTumor Cell Resistance to Apoptotic Stresses. Gastroenterology 132(7): 2459-77 [Pubmed]

PERK-dependent compartmentalization of ERAD and unfolded protein response machineries during ER stress. Experimental Cell Research, 313(16):3395-407. [Pubmed]

Statin- mediated cytoprotection of human vascular endothelial cells: a role for Kruppel-like factor 2-dependent induction of heme oxygenase-1. Ali F, Hamdulay SS, Kinderlerer AR, Boyle JJ, Lidington EA, Yamaguchi T, Soares MP, Haskard DO, Randi AM, Mason JC: J Thromb
Haemost 2007; 5: 2537–46.

A decrease in cellular energy status stimulates perk-dependent eIF2α phosphorylation and regulates protein synthesis in pancreatic β-cells. Edith Gomez, Mike L Powell, Alan Bevington and Terence P Herbert. Biochem. J. (2007) Immediate Publication, doi:10.1042/BJ20071367

We have found the microporator ideal for transfection of dissociated neurons. Transfection efficiencies are at least as good as those achieved with lipofection, biolistic transformation or other electoporators. In addition, the very small volumes, and hence cell numbers, required for the microporator means that from a practical and ethical point of view this is the machine of choice for neuronal transfection in our lab.
Professor John Wood
Neurobiology
University College London

 
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