MP-100 Microporator - pipette tip based electroporator
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Why does Microporation show such fantastic transfection effiency? Prior to Microporation, all electroporation took place in cuvette style chambers. Microporation is a unique electroporation technology using a novel pipette tip as an electroporation space. In doing so, a uniform electric field is generated accompanying minimal heat generation, metal ion dissolution, pH variation and oxide formation, which are harmful events to the cell during conventional electroporation process.

Conventional Cuvette: Poor uniformity of electric field, Al3+ production, oxide formation, water dissociation and Hydrogen evolution, local pH changes and excess heat generation all combine to cause poor cell viability and low transfection efficiency

The Microporator Gold-Tp

The unique design of the Gold-Tip increases the Length / Depth ratio of the electroporation cell providing an optimal environment for cell transformation

  • High transfection efficiency and cell survival rate
  • Uniform electric field
  • Excellent heat dissipation (10 µl)
  • Low pH variation
  • Low metal ion effect
  • Integration of sample handling and electroporation - true aseptic transformations
  • Small (10 µl) or large (100 µl) sample volume
  • Easy handling and operation
  • High throughput compatible

Correation between transfection efficiency and length / depth ratio

 

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