NanoDrop Nd-1000 UV/Vis 1µl Spectrophotometer
BioTek

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The NanoDrop® ND-1000 Spectrophotometer can be used to measure protein concentration via the BCA (Bicinchoninic Acid) Protein Assay. This assay is often used for more dilute protein solutions and/or for proteins in the presence of components that also have significant UV (280 nm) absorbance. The resulting Cu-BCA chelate formed in the presence of protein is measured at its at its wavelength maximum of 562 nm. The BCA Assay requires that a standard curve be generated before unknown proteins can be measured.

Key Features

  • Both Regular and Mini BCA assays can be run
  • Allows generation of standard curves with up to 7 standards. Four curve fitting analysis options available
  • Concentration of protein is calculated and displayed
  • Reblank feature allows for the re-analysis of previous sample with a new blank
  • Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
  • Sample ID field is key-focused for easy bar coder use
  • Absorbance is represented as a 1mm path equivalent

Measurement concentration range

Assay Type
Detection Limit (mg/ml)
Approximate Upper Limit (mg/ml)
Typical Reproducibility
(minimum 5 replicates)
(SD= mg/ml; CV= %)
Regular BCA
0.2
8.0
± 2% (over entire range)

Mini BCA
0.01
0.20
± 0.01 mg/ml (over entire range)

 
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