| The NanoDrop® ND-1000 Spectrophotometer can be used to measure protein concentration via the Bradford Assay. This method is often used for more dilute protein solutions where lower detection sensitivity is needed and/or in the presence of components that also have significant UV (280 nm) absorbance. The Bradford uses the protein-induced Absorbance shift of Coomassie Blue dye to 595 nm as a measure of protein concentration and requires standard curve generation each time the assay is run. |
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Key Features
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Both Regular and Mini Bradford assays can be run
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Allows generation of standard curves with up to 7 standards. Four curve fitting analysis options available
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Concentration of protein is calculated and displayed
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Reblank feature allows for the re-analysis of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
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Sample ID field is key-focused for easy bar coder use
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Absorbance is represented as a 1mm path equivalent
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Measurement concentration range
Assay Type |
Detection Limit (ug/ml) |
Approximate Upper Limit (ug/ml) |
Typical Reproducibility
(minimum 5 replicates)
(SD= ug/ml; CV= %) |
Regular Bradford |
100 |
8000 |
sample range 100-500 ug/ml: ± 25 ug/ml
sample range 500-8000 ug/ml: ± 5%
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|
15 |
125 |
sample range 15-50 ug/ml: ± 4 ug/mlsample range 50-125 ug/ml: ± 5% |
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ND-1000 Applications - Bradford Assay 