BCA Assay
The NanoDrop® ND-8000 Spectrophotometer can be used to measure protein concentration via the BCA (Bicinchoninic Acid) Protein Assay. This assay is often used for more dilute protein solutions and/or for proteins in the presence of components that also have significant UV (280 nm) absorbance. The resulting Cu-BCA chelate formed in the presence of protein is measured at its at its wavelength maximum of 562 nm. The BCA Assay requires that a standard curve be generated before unknown proteins can be measured.
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Both Regular and Mini BCA assays can be run
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Allows generation of standard curves with up to 7 standards. Four curve fitting analysis options available
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Concentration of protein is calculated and displayed
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Reblank feature allows for the re-analysis of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
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Sample or plate ID can be preloaded by using a barcode reader or importing from a file
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Absorbance is represented as a 1mm path equivalent
Measurement concentration range
Assay Type |
Detection Limit (mg/ml) |
Approximate Upper Limit (mg/ml) |
Typical Reproducibility
(minimum 5 replicates)
(SD= mg/ml; CV= %) |
Regular BCA |
0.2 |
8.0 |
± 2% (over entire range)
|
|
0.01 |
0.20 |
± 0.01 mg/ml (over entire range) |
