Bradford Assay

The NanoDrop^® ND-8000 Spectrophotometer can be used to measure protein concentration via the Bradford Assay. This method is often used for more dilute protein solutions where lower detection sensitivity is needed and/or in the presence of components that also have significant UV (280 nm) absorbance. The Bradford uses the protein-induced Absorbance shift of Coomassie Blue dye to 595 nm as a measure of protein concentration and requires standard curve generation each time the assay is run.

• Both Regular and Mini Bradford assays can be run
• Allows generation of standard curves with up to 7 standards. Four curve fitting analysis options available
• Concentration of protein is calculated and displayed
• Reblank feature allows for the re-analysis of previous set of samples with a new set of references (blanks) for each channel
• Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
• Sample or plate ID can be preloaded by using a barcode reader or importing from a file
• Absorbance is represented as a 1mm path equivalent

Measurement concentration range