NanoDrop ND-8000 Eight Sample UV/Vis 1µl Spectrophotometer
BioTek

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bullet on Microarray sample measurement

The capability to pre-select viable fluorescent-tagged hybridization probes for gene expression in microarrays can eliminate potentially flawed samples and improve research effectiveness. The NanoDrop software facilitates the measurement of DNA concentration and dye labeling effectiveness. The NanoDrop® ND-8000 Spectrophotometer measures the absorbance of the fluorescent dye, allowing detection at dye concentration as low as 0.2 pmol/ul.

  • Includes analysis capability for dsDNA, RNA and "other" (analysis constant selectable between 15-55)
  • Measurement of wide variety of fluorescent dyes possible: Cy3, Cy5, Cy3.5, Cy5.5, Alexas 488, 546, 555, 594, 647 and 660
  • Concentration of nucleic acid and dye calculated and displayed
  • Reblank feature allows for the re-analysis of previous set of samples with a new set of references (blanks) for each channel
  • Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
  • Sample or plate ID can be preloaded by using a barcode reader or importing from a file
  • 260/280 ratio calculated and displayed
  • Absorbance is represented as a 1mm path equivalent

Measurement Concentration Range

Sample Type
Detection Limit
(pmol/ul)
Approximate Upper Limit (pmol/ul)
Typical Reproducibility
(minimum 5 replicates)
(SD= pmol/ul; CV= %)

Cy3, Cy3.5, Alexa Fluor 555, Alexa Fluor 660
0.20
100
sample range 0.20-4.0 pmol/ul: ± 0.20 pmol/ul sample range >4.0 pmol/ul: ± 2%

Cy5, Cy5.5 and Alexa Fluor 647
0.12
60
sample range 0.12-2.4 pmol/ul: ± 0.12 pmol/ul sample range >2.4 pmol/ul: ± 2%

Alexa Fluor 488 and Alexa Fluor 594
0.40
215
sample range 0.40-8.0 pmol/ul: ± 0.40 pmol/ul sample range >8.0 pmol/ul: ± 2%

Alexa Fluor 546
0.30
145
sample range 0.30-6.0 pmol/ul: ± 0.30 pmol/ul sample range >6.0 pmol/ul: ± 2%
 
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