NanoDrop ND-8000 Eight Sample UV/Vis 1µl Spectrophotometer
BioTek

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The NanoDrop® ND-8000 Spectrophotometer can be used to simultaneously measure the concentrations of proteins (A280) and up to two fluorescent dyes. In addition to measuring both components of a protein-dye conjugate, the versatile Proteins and Labels module can also be used to measure the purity of metalloproteins (such as hemoglobin) using wavelength ratios.

  • Six different reference types available : 1 abs=1mg/ml, BSA, IgG, Lysozyme, user entered mass extinction coefficient, user entered molar extinction coefficient
  • Measurement of wide variety of fluorescent dyes possible: Cy3, Cy5, Cy3.5, Cy5.5, Alexas 488, 546, 555, 594, 647 and 660
  • Concentration of protein and dye calculated and displayed
  • Reblank feature allows for the re-analysis of previous set of samples with a new blank
  • Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
  • Sample or plate ID can be preloaded by using a barcode reader or importing from a file
  • Absorbance is represented as a 10mm path equivalent

Measurement Concentration Range

Sample Type
Detection Limit
(pmol/ul)
Approximate Upper Limit (pmol/ul)
Typical Reproducibility
(minimum 5 replicates)
(SD= pmol/ul; CV= %)

Cy3, Cy3.5, Alexa Fluor 555, Alexa Fluor 660

0.20
100
sample range 0.20-4.0 pmol/ul: ± 0.20 pmol/ul sample range >4.0 pmol/ul: ± 2%

Cy5, Cy5.5 and Alexa Fluor 647

0.12
60
sample range 0.12-2.4 pmol/ul: ± 0.12 pmol/ul sample range >2.4 pmol/ul: ± 2%

Alexa Fluor 488 and Alexa Fluor 594

0.40
215
sample range 0.40-8.0 pmol/ul: ± 0.40 pmol/ul sample range >8.0 pmol/ul: ± 2%

Alexa Fluor 546

0.30
145
sample range 0.30-6.0 pmol/ul: ± 0.30 pmol/ul sample range >6.0 pmol/ul: ± 2%

 

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