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NanoDrop ND2000

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The Thermo Scientific NanoDrop 2000 is the only
micro-volume spectrophotometer with a patented
sample retention technology that allows for sample volumes as small as 0.5 µL. It offers:
  • Direct, easy measurements in less than 5 seconds – just
    pipette & wipe
  • Full spectral output
  • Measures DNA, RNA (A260) and Protein (A280)
    concentrations and sample purity (260/280 ratio)
  • Large concentration range (2 ng/µL – 15,000 ng/µL dsDNA) without dilutions
  • Pre-configured methods for common applications such as
    Nucleic Acid, Protein A280, Microarray, Proteins & Labels,
    Bradford, BCA, Lowry and Pierce 660nm – and more
  • User-friendly software that includes Custom Methods and
    data export capabilities
  • Simple self calibration check using control fluid – no
    instrument adjustments
  • Low-cost operation – no plates or other consumables
How It Works
 
Pedestal Measurements
The patented sample retention system used by the Thermo Scientific
NanoDrop 2000 and NanoDrop™ 2000c allows for the analysis of
0.5 µl - 2.0 µl samples, without the need for cuvettes or capillaries.

With the arm open, a sample is pipetted directly onto the pedestal.
After the arm is closed, a sample column is formed.
The pedestal then moves to automatically adjust for an optimal path
length (0.05 mm - 1 mm).
When the measurement is complete, the surfaces are simply wiped with a
lint-free lab wipe before going on to the next sample.
Performance
Data from studies using the Thermo Scientific NanoDrop 2000 spectophotometer are below:

 
 
Specifications
Instrument Type  
Spectrophotometer
Minimum Sample Size
0.5 µl
Sample Number
1
Path Length
1 mm (auto-ranging to 0.05 mm)
Light Source(s)
Xenon flash lamp
Detector Type
2048 - element linear silicon CCD array
Wavelength Range
190 - 840 nm
Wavelength Accuracy
1 nm
Spectral Resolution
< 1.8 nm (FWHM at Hg 253.7 nm)
Absorbance Precision
0.002 (1 mm path)
Absorbance Accuracy
2% (at 0.76 at 257 nm)
Absorbance Range
0.02 - 300 (10 mm equivalent)
Detection Limit
2 ng/µl (dsDNA)
Maximum Concentration
15,000 ng/µl (dsDNA)
Measurement Time
< 5 seconds
Footprint
14 x 20 cm
Weight
2.0 kg
Sample Pedestal Material of Construction
303 stainless steel and quartz fiber
Operating Voltage
12 vdc
Operating Power Consumption
12 - 18 W (max 30 W)
Standby Power Consumption 
5 W
Software Compatibility
Microsoft Windows XP (32 bit) with service pack (SP) 2 or later, Windows Vista (32 bit)
 
 
Software
Classic NanoDrop Applications
  • Nucleic Acid
  • Protein A280
  • Micro Array
  • Proteins & Labels
  • BCA, Bradford, Lowry,
    Pierce 660 nm
  • UV-Vis
  • Cell Cultures
  • plus Diagnostics
New Applications
  • User defined custom methods
New and updated
  • Intuitive user interface
  • Flexible reporting & data export
  • Oligo calculator, units flexibility & more