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NanoDrop ND2000c

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NanoDrop 2000c

Dual-mode UV-Vis Spectrophotometer with Micro-Volume AND
Cuvette Capability for Nucleic Acid and Protein Quantitation

The Thermo Scientific NanoDrop 2000c is the only
micro-volume spectrophotometer that integrates a patented sample retention technology for samples as
small as 0.5 µL and a cuvette capability.

  • All the features of the NanoDrop 2000™ Spectrophotometer
    – plus cuvette mode
  • Pre-configured methods for all common Nucleic Acid and
    Protein Concentration and Purity measurements, in both modes
  • Custom Methods capability, in both modes
  • Cell cultures (OD600)
  • Kinetic studies (time and 37°C temp) with stirring, in
    cuvette mode
  • Extended concentration range (lower limit 0.4 ng/µL dsDNA)
    in cuvette mode
NanoDrop 2000c in the Laboratory

NanoDrop 2000c loading with the pippette
NanoDrop 2000c loading a cuvette
Micro-Volume Pedestal
Accepts Standard Cuvettes

How It Works

Pedestal Measurements

The patented sample retention system used by the Thermo Scientific
NanoDrop 2000 and NanoDrop™ 2000c allows for the analysis of
0.5 µl - 2.0 µl samples, without the need for cuvettes or capillaries.

With the arm open, a sample is pipetted directly onto the pedestal.





After the arm is closed, a sample column is formed.




The pedestal then moves to automatically adjust for an optimal path
length (0.05 mm - 1 mm).




When the measurement is complete, the surfaces are simply wiped with a
lint-free lab wipe before going on to the next sample.

Cuvette Measurements

The NanoDrop 2000c model includes a built-in cuvette capability. Most
common cuvettes and micro-cuvettes may be used with this instrument
(8.5 mm z-height and 2 mm beam).

Specifications

Instrument Type   Spectrophotometer  
Minimum Sample Size 0.5 µl
Sample Number 1
Path Length 1 mm (auto-ranging to 0.05 mm)
Light Source(s) Xenon flash lamp
Detector Type 2048 - element linear silicon CCD array
Wavelength Range 190 - 840 nm
Wavelength Accuracy 1 nm
Spectral Resolution < 1.8 nm (FWHM at Hg 253.7 nm)
Absorbance Precision 0.002 (1 mm path)
Absorbance Accuracy 2% (at 0.76 at 257 nm)
Absorbance Range 0.02 - 300 (10 mm equivalent)
Detection Limit 2 ng/µl (dsDNA)
Maximum Concentration 15,000 ng/µl (dsDNA)
Measurement Time < 5 seconds
Footprint 14 x 20 cm
Weight 2.1 kg
Sample Pedestal Material of Construction 303 stainless steel and quartz fiber
Operating Voltage 12 vdc
Operating Power Consumption 12 - 18 W (max 30 W)
Standby Power Consumption  5 W
Software Compatibility Microsoft Windows XP (32 bit) with service pack (SP) 2 or later, Windows Vista (32 bit)
   
Cuvette specifications  
   
Z-Height 8.5 mm
Heating 37 ± 0.5 °C
Stirrer: 150-850 rpm
Path Length 10, 5, 2, 1 mm
Absorbance Range 0.002 - 1.5
Detection Limit 0.4 ng/ul (dsDNA)
Maximum Concentration 750 ng/µl (dsDNA)
Measurement Time < 3 seconds
Cuvette Dimensions 12.5 mm x 12.5 mm, up to 48 mm H
Type Masked cuvette
UL/CSA and CE All units are approved to these standards

Performance

Data from studies using the Thermo Scientific NanoDrop 2000c spectophotometer are below:

Software
Classic NanoDrop Applications
  • Nucleic Acid
  • Protein A280
  • Micro Array
  • Proteins & Labels
  • BCA, Bradford, Lowry,
    Pierce 660 nm
  • UV-Vis
  • Cell Cultures
  • plus Diagnostics
New Applications
  • User defined custom methods
New and updated
  • Intuitive user interface
  • Flexible reporting & data export
  • Oligo calculator, units flexibility & more