Using our patented sample retention technology, the versatile and cost-effective Thermo Scientific NanoDrop 3300 Fluorospectrometer makes broad spectrum fluorescence analysis quick and easy. It offers:
Sample size as small as 1 µl
Mass detection limit more than one order of magnitude lower than conventional fluorometers
UV, Blue and White LED excitation, covering a broad wavelength range
No filter changes, no monochromator
Ability to analyze multiple emission profiles from a single sample
A cost-effective, unique alternative to high-end fluorometers or complicated lab-built systems with microscopes and CCD cameras
How It Works
The sample retention system used by the Thermo Scientific NanoDrop 3300 Fluorospectrometer enables analysis of extremely small sample volumes (1-2 µl) without the use of cuvettes or capillaries. Preparing the instrument for another measurement simply involves cleaning the sample off with an ordinary lab wipe. The system saves on expenses associated with disposables as well as on time and effort spent cleaning quartz cuvettes.
With the apparatus arm open, a droplet of sample is pipetted onto the measurement pedestal.
When the apparatus is closed, the sample arm slightly compresses the droplet and a liquid column is drawn. Surface tension alone holds the sample in place for the spectral measurement. The spectral measurement is then made and quantification is made based a sample length of 1 mm.
When the measurement is complete, the apparatus arm is opened and the sample is simply wiped from both the upper bushing and lower pedestal using an ordinary dry laboratory wipe.
Since the sample is not contained in a secondary vessel, the sample directly wets the system optics, reducing the variations resulting from changing and/or repositioning cuvettes. When the sample is removed, the optics can be easily cleaned making it possible to measure successive sample concentrations varying by more than 1000 fold in concentration with no carryover.
Specifications
Instrument Type
Fluorospectrometer
Minimum Sample Size
1 µl
Light Sources
3 light emitting diodes (LEDs)
Excitation Maxima
UV: 365 nm Blue: 470 nm White 460-650 nm
Detector Type
2048 - element linear silicon CCD array
Wavelength Range
400-750 nm
Wavelength Accuracy
1 nm
Spectral resolution
8 nm (FWHM at Hg 546 nm)
Fluorescence Precision
< 5% CV (10 nM fluorescein)
Detection Limit
< 1 fmol fluorescein
Measurement Cycle Time
2 - 10 seconds
Dimensions (footprint)
14 cm X 20 cm
Weight
1.5 kg
Sample Pedestal Material of Construction
303 stainless steel and quartz fiber
Operating Voltage
5 vdc (supplied by USB port, no external power supply)
Operating Power Consumption
2 W
Standby Power Consumption
1 W
Software Compatibility
Microsoft Windows® 2000, XP, and Vista (32 bit)
UL/CSA and CE
All units are approved to these standards
Performance
Data from studies using the Thermo Scientific NanoDrop 3300 fluorospectrometer on many fluorophores is available below:
