NanoDrop 8000
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Multi-Sample Micro-Volume UV-Vis Spectrophotometer
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With the Thermo Scientific NanoDrop 8000, you can measure more samples in less time with the same
reliability and patented sample retention technology of
the popular NanoDrop™ 2000. It offers:
- Measure nucleic acid and protein concentration of samples as small as 1 µL
- Direct, easy measurements from tubes or plates
- Single sample mode or up to eight samples at a time
- Analysis of 96 samples in less than 6 minutes
- Full spectral output
- Calculates sample purity ratios (260/280)
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Pre-configured methods for common applications such as Nucleic Acid, Protein A280, Proteins Microarray, Proteins & Labels, Bradford, BCA, Lowry and Pierce 660nm – and more
- Intuitive and flexible software for creating custom methods
and exporting data
- No consumables
- Sample Position Illuminator which increases efficiency and reduces error by keeping track of the samples to be measured
Perfect for core labs, bioprocessing QC/manufacturing environments, biorepositories and molecular diagnostics labs.
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Sample Position Illuminator |
Multi-Sample Loading |
Multi-Sample Measurement |
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How It Works
The 8-sample retention system used by the Thermo Scientific NanoDrop 8000 spectrophotometer is one of the key features of the instrument. Eight samples are simultaneously captured between an array of upper and lower optical surfaces, eliminating the need for cuvettes and capillaries. This saves on the cost of disposable cuvettes and/or on the time and effort spent in cleaning reusable ones.
| With the sample apparatus open, eight 1 µl samples are pipetted directly onto the array of optical surfaces. |
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When the sample apparatus is closed, the sample arm slightly compresses the droplets and a sample column is formed. Surface tension alone holds the samples in place.
A spectral measurement and quantification of each sample is made based on the tightly controlled path length. |
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When the measurements are complete, the sample apparatus is opened and the samples are simply wiped from both upper and lower optical arrays with an ordinary dry laboratory wipe.
Since the samples are not contained in a secondary vessel, the samples directly wet the system optics, reducing the variations resulting from changing and/or repositioning cuvettes. When the samples are removed, the optics can be easily cleaned making it possible to measure successive sample varying by more than 1000 fold in concentration with no carryover.
Specifications
| Sample size |
1 µl |
| Sample Number |
up to 8 |
| Path lengths |
1 mm and 0.2 mm |
| Light Source |
Xenon flash lamp |
| Detector Type |
2048-element linear silicon CCD |
| Wavelength range |
220-750 nm |
| Wavelength accuracy |
1 nm |
| Wavelength resolution |
3 nm (FWHM at Hg 546 nm) |
| Absorbance Precision |
0.003 absorbance (1 mm path) |
| Absorbance Accuracy |
2% (at .76 absorbance at 257 nm) |
| Absorbance Range |
0.02-75 (10 mm equivalent absorbance) |
| Detection Limit |
2 ng/µl (dsDNA) |
| Maximum Concentration |
3700 ng/µl (dsDNA) |
| Measurement Cycle Time |
20 seconds (for 8 samples) |
| Dimensions (footprint) |
24 cm X 32 cm |
| Weight |
3.4 kg |
| Sample Pedestal Material of Construction |
303 stainless steel and quartz fiber |
| Operating voltage |
12 vdc |
| Operating Power Consumption |
30 W |
| Standby Power Consumption |
6 W |
| Software Compatibility |
Microsoft Windows® 2000, XP, and Vista (32 bit) |
| UL/CSA and CE approval |
All units |
Performance
Data from studies using the Thermo Scientific NanoDrop 8000 Spectrophotometer are below:
Software
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Pre-configured applications
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Nucleic Acid
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Protein A280
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Micro Array
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Proteins & Labels
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BCA, Bradford, Lowry, Pierce 660
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UV/Vis
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Cell Cultures
Additional capabilities
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User defined custom methods
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Oligo calculator, units flexibility & more
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Sample Position Illuminator |
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Position Illuminator
When measuring samples in a 96-well format on the Thermo Scientific
NanoDrop 8000 Spectrophotometer, an eight-channel pipettor is used
to draw 1 ul samples from a microtiter plate which are then dispensed
onto the eight optical measurement surfaces.
The Sample Position Illuminator keeps track of the samples to be measured
by lighting up wells from beneath the plate. This helps the user readily identify which wells to draw from for the next measurement, thus reducing fatigue and human error.
The sample position illuminator is simple to use. Prior to measurement, the
user selects the samples to be measured from the on screen position indicator (selected wells are in green).
The sample position illuminator then lights up the corresponding wells of
the plate that are to be measured:
The user then simply draws from the illuminated wells.
Additional Features:
- Auto advance: This increases measurement throughput by automatically
lighting up the next column to be measured.
- "Cherry picking": User can select any single or multiple wells which are
then lit up by the sample position illuminator.
- Measurement limits: The user can define upper and lower concentration
(or absorbance) limits such that a well will begin to flash if the
measurement falls out of adefined range (e.g. 100ng/ul ±20ng/ul).
