PCR Reagents

Phusion DNA Polymerase Finnzymes’ Phusion™ DNA Polymerases offer PCR performance that no other enzyme can match. They combine the fidelity you demand with unparalleled speed and robustness. Phusion High-Fidelity DNA Polymerases are part of Finnzymes' High Performance PCR!
Phusion Products Phusion™ Hot Start High-Fidelity DNA Polymerase (F-540) Phusion™ High-Fidelity DNA Polymerase (F-530) Phusion™ Flash High-Fidelity PCR Master Mix (F-548) Phusion™ High-Fidelity PCR Master Mix (F-531) Phusion™ High-Fidelity PCR Kit (F-553) Phusion™ Site-Directed Mutagenesis Kit (F-541) Phusion™ RT-PCR Kit (F-546) Buffer packs for Phusion™ DNA Polymerases
Phire Hot Start DNA Polymerase Speed and specificity for routine and high-throuput PCR
Finnzymes’ Phire™ Hot Start DNA Polymerase is a novel PCR enzyme that outperforms every Taq-based hot start polymerase on the market. This is a result of the polymerase’s DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, our unique hot start technology allows complete reactivation of Phire Hot Start DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymerase an ideal solution for routine and high throughput PCR applications. Phire DNA Polymerase delivers superior performance in all thermal cyclers, both conventional and fast instruments such as the Piko™ Thermal Cycler from Finnzymes.

DNA Polymerase Selection Guide

	Phusion™ Flash High-Fidelity DNA Polymerase	Phusion™ Hot Start High-Fidelity DNA Polymerase	Phusion™ High-Fidelity DNA Polymerase	Phire™ Hot Start DNA Polymerase	DyNAzyme™ EXT DNA Polymerase	DyNAzyme™ II Hot Start DNA Polymerase	DyNAzyme™ II DNA Polymerase	DyNAzyme™ I DNA Polymerase
Fast PCR	+++	+++	+++	+++	+	+	+	+
High-fidelity applications	+++	+++	+++	+	++	+	+	+
Cloning	+++1	+++1	+++1	+1	++	+	+	+
Difficult templates	+++	+++	+++	++	+++	++	++	++
Long-range PCR	+++	+++	+++	+++	+++	+	++	++
Routine PCR	++	++	++	+++	+++	+++	+++	+++
Microarray2	+	+	+++	+	+++	++	++	++
Site-directed mutagenesis3	+++	+++	+++	+	++	+	+	+
DHPLC2	+	+	+++	+	+++	++	++	++

1. Phusion and Phire DNA polymerases generate blunt end PCR products.
Click here for cloning instructions for Finnzymes' DNA Polymerases
2. Detergent-free reaction buffers are available separately for microarray and DHPLC applications, where PCR products are recommended to be free of detergents.
Click here for more information on Polymerase Buffers
3. For site directed mutagenesis, Finnzymes offers a complete kit:
Phusion™ Site-Directed Mutagenesis Kit