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PA-3000S Laboratory PulseAgile® Electroporation System
Budget-conscious researchers looking to immediately improve gene transfer or siRNA knockdown abilities can start fast and easy with the PA-3000. After minimal equipment setup, you'll be ready to process your cell lines in no time!
Rectangular wave electroporators have become industry standard because of their ability to increase transfection efficiency while maintaining viability over older and less
The Cyto Pulse Sciences patented PulseAgile® protocol series takes the concept of square wave electroporation one step further: users are given the choice to create multiple protocols with different parameters, called groups, that are run in direct succession of one another. This is generally used to deliver a series of high voltage and short duration pulses first permeabilizing the cell membrane, followed by a series of low voltage and long duration pulses to continue delivery of the transfectant by electrophoresis.
The ultimate goal of creating these types of protocols is to deliver similar transfection efficiencies while retaining an even higher viability than standard four-parameter waves. In particular, published in-vivo applications have repeatedly been found to substantially improve delivery in addition to some ex-vivo applications.
There is no mystery to what ís inside the box when all necessary information can be accessed from the outside. Included as standard features are one each of voltage and current monitors ready to attach with an external oscilloscope in order to view real-time pulsing conditions. Using these connections is a true safeguard to know exactly how the applied waveform reflects the programmed waveform, so you never again have to wonder if your protocol ran as intended. Furthermore, an output load estimate is provided after every run to assist in understanding how ionic the pulsing conditions were. This can be extremely valuable when debugging unexpected results due to incorrect sample washing for ex-vivo cuvette applications or proper electrode insertion for in-vivo applications. Information relevant to each executed protocol may be saved as a log file, including actual voltage levels of all pulses.
All Cyto Pulse Sciences equipment is designed to withstand extreme user conditions. In the event of a fault, advanced control circuitry immediately detects the source of any problem and proceeds to halt the system to prevent permanent damage. The safety of Cyto Pulse customers is our number one priority.
Cyto Pulse also offers parallel row needle in vivo electrodes for use with PA-3000 and PA-4000 based systems.
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Currency:
- 3-D Cell Culture
- Cell counting
- Cell Imaging
- Cold Storage
- Disposable Hemocytometers
- Electroporation
- FISH Probes
- Fluorometers
- High performance PCR
- Image analysis/Gel doc
- Liquid handling
- Luminescence reagents
- Luminometers
- Microplate readers
- Microscopes
- Multi-Detection Readers
- Patch Clamping
- Software
- Spectrophotometers
- Surface Plasmon Resonance
- Transfection
Rectangular (square) Wave
PulseAgile
The heavy-duty electroporation cuvette holder will operate with all major cuvette manufacturers, although Cyto Pulse cuvettes are recommended for best results; standard cuvette sizes are available in 1 mm, 2 mm, and 4 mm electrode gaps. The insertion mechanism is notched to prevent incorrect positioning and only upon full insertion will high voltage become enabled to pulse. A large plastic barrier surrounds the cuvette field to completely block any contact with the electrodes.