Labtech International Ltd. - SensiQ® Semi-automated, dual channel surface plasmon resonance

SensiQ® Semi-automated, dual channel surface plasmon resonance

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SensiQ®: Overview

Cost Effective, Surface Plasmon Resonance System for Biomolecular Interaction Analysis

Surface plasmon resonance has proved to be an invaluable tool for scientists involved in characterizing biomolecular interactions for a wide range of research activities such as antibody selection and screening, drug discovery, ligand fishing, binding specificity, and gene regulation. However, researchers have long been dependent on core facilities and centralized labs for access to SPR technology. Now, the ability to perform binding interactions in real time and obtain high quality affinity and kinetic data is available to labs of all sizes in an uncompromised, affordable solution.

SensiQ Explorer is a dual-channel, semi-automated SPR system which utilizes advanced microfluidics, proven surface attachment methodologies, and state-of-the-art data analysis tools to provide kinetic, affinity and concentration data researchers can use with a high degree of confidence. SensiQ has been designed with the end-user in mind through the use of control and data handling wizards to insure ease of operation through the entire experimental processs. With innovative hardware and powerful software tools, SensiQ will set a new standard for biomolecular interaction analysis.

SensiQ®: Features

SensiQ features many of the key benefits of more costly instruments and improves upon them in many ways.

High quality affinity and kinetic data at an affordable price.
Real-time reference subtraction achieved through low dead-volume microfluidics.
High mass transport of analyte to the sensor surface using low nanoliter volume flow channels.
Easy to use control software utilizing software wizards.
Integrated, proven data analysis software based on the Scrubber and Clamp platforms.
High sensitivity achieved through a robust, stable design based on Kretschmann optics.
Wide variety of surface chemistries available to handle almost any application.
Low noise and drift achieved through Peltier temperature control.

SensiQ®: Applications

As a technique for studying biomolecular binding, surface plasmon resonance has been shown to be of great value in a diverse range of research activities including drug discovery, cell signaling, gene regulation, and structure-function studies.

Some of the different types of affinity pairs to be characterized by this technique include:

Protein-Protein
Protein-DNA
Protein-Small Molecule
Antibody-Antigen
Membrane receptor-Ligand
Peptide-Receptor

Kinetics of association and dissociation, affinity constant measurements, concentration determinations and ligand fishing are all examples of the capabilities inherent in SPR and SensiQ provides this valuable data in an easy to use, affordable instrument.

SensiQ®: Specifications

Technical Specifications
Real time reference curve subtraction	Yes
Channel to channel dead volume	< 60nl
Flow cell volume	10 to 250µl
Refractive index range	1.33-1.40
Short time noise	< 0.25RU
Long term noise	< 0.40RU/min
Flow rate	5-150 µl/min
Sample loading	manual (syringe)
Sample inject	automatic
Temperature control	15°-40°C; (+15° / -5° from ambient)

Working Ranges
k_a	1 x 10⁸M^-1s^-1
k_d	10^-1-10^-6s^-1
Concentration	~10^-3 - 10^-11M
K_D	~10^-4 - 10^-11

SensiQ®: Surface Chemistries

ICx Nomadics Recommendation for Kinetic Analysis Applications - We recommend two dimensional surface chemistry for kinetic applications as the interpretation of kinetic constants is simplified.

1. Gold Only Surface

Sensor provided with gold coated glass surface only to facilitate the attachment of user-developed functional chemistry.

2. COOH1

This general purpose, two dimensional coating is suitable for a wide variety of biomolecular interactions and has proven to resist non-specific binding better than any other coating. Coupling of the ligand to the surface is normally achieved via amine coupling(EDC/NHS chemistry) but alternative methods such as thiol coupling, disulfide exchange and hydrazide coupling may also be employed.

3. COOH2

This modified, carboxylate surface is very similar to the COOH chemistry and may be used with the same coupling protocols. In some applications, this surface may reduce non-specific binding when compared to the standard COOH surface chemistry.

4. BioCap

This is a two dimensional coating that utilizes the high affinity biotin-neutravidin interaction as the basis for immobilization of ligands. It is ideal for the capture of biotinylated reagents including peptides, oligonucleotides, proteins and sugars in a non-reversible manner.

5. VesCap

The VesCap surface chemistry is a two dimensional chemistry for the immobilization of vesicles. Liposomes, unilamellar vesicles and even crude membrane preparations may be conveniently immobilized in a single step. Components embedded in the vesicles will continue to diffuse and this property makes this surface ideal for membrane bound receptor studies.

6. HisCap

The HisCap surface is a two dimensional chemistry for the immobilization of His-tagged ligands.. This surface is ideal for reversible capture of polyhistidine-tagged recombinant proteins. Immobilized proteins may be removed by exposure to imidazole allowing the surface to be re-coated.

7. HiCap

This is a three dimensional, polymeric surface chemistry which is ideal for amine coupling of small peptides, drugs and other small molecules that possess amine groups. Such biomolecules show negligible preconcentration and must be exposed to the surface at high concentrations... The lack of charge confers high resistance to non-specific binding. Very high binding capacities may be achieved.

8. AvCap

This is a three dimensional polymeric coating utilizing biotin to capture avidin-conjugated ligands. It is a non-charged thereby limiting non-specific binding when compared to other negatively charged surfaces. It provides high capacity immobilization and is ideal for acidic proteins or where high binding capacity is required.