Electroporation and Electrofusion Protocols

ex vivo transfection

Cell Line	eGFP	Luciferase	mRNA	siRNA	Other
293
3T3-L1 Pre-adipocyte
A20
CHO-K1
Dendritic (DC), Human
Dendritic (DC), Mouse
E Coli
DBS-FRhL-2
HeLa
Jurkat
K-562
Neuro-2a
PC-12
RAW264.7

ex vivo cell fusion

Immunotherapy
	• Human dendritic cells and A549 cell line (66k size)
Hybridoma production
Nuclear transfer
	• Mouse embryo (62k size)

In vivo transfection

Pig skin (61k size)

Mouse larynx (71k size)

Mouse melanoma tumor (61k size)

In vivo transfection

Mouse Skin (57k size)

Transdermal Delivery

TBA