Finnzymes: High Performance PCR

PCR Reagents
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bullet off Phusion™ Flash High-Fidelity DNA Polymerase
bullet off Phusion™ Hot Start High-Fidelity DNA Polymerase
bullet off Phusion™ High-Fidelity DNA Polymerase
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Piko & Slidetiter demonstration movie high resolution (15.2MB)

bullet on Phusion™ Flash High-Fidelity PCR Master Mix

2x Master Mix developed especially for fast high-fidelity PCR.

Description

Phusion™ Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilizes Phusion Flash DNA Polymerase, a proofreading DNA polymerase modified from Phusion Hot Start High-Fidelity DNA Polymerase.

The error rate when using Phusion Flash PCR Master Mix is 9.5 x 10-7, i.e. the fidelity of the Phusion Flash DNA Polymerase is 25 x Taq.

Extreme speed and high yields

Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use master mix minimizes required pipetting steps: Only template and primers need to be added by the user. In addition, the unique composition of the master mix enables the use of extremely short cycling protocols with both low and high complexity DNA templates – 15 seconds per kilobase or less.

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Comparison of performance
versus extension times		 Comparison of PCR cycling times

Storage and stability

Recommended storage temperature -20°C. Stable for six months from the packaging date

Ordering information

Phusion™ Flash High-Fidelity PCR Master Mix

F-548S

100 reactions à 20 µl (1.0 ml)

F-548L

500 reactions à 20 µl (5 x 1.0 ml)