Complete kit with extreme performance for all PCR applications
Description
Phusion™ High-Fidelity PCR Kit is available in two sizes. The small kit
(F-553S) contains all the necessary reagents for performing 50 amplifications in 50 µl volume or 125 amplifications in 20 µl volume. The large kit contains reagents for 200 amplifications in 50 µl volume or 500 amplifications in 20 µl volume. The kit also contains control template (λ DNA) and two primer sets (1.3 kb and 10 kb) for performing 20 control reactions in 50 µl volume or 50 control reactions in 20 µl volume.
Storage and stability
Recommended storage temperature -20°C.
Stable for one year from the packaging date.
Phusion™ High-Fidelity PCR Kit components
Component |
Concentration |
F-553S |
F-553L |
Phusion DNA Polymerase |
2 U/µl |
50 U |
200 U |
5x Phusion™ HF Buffer |
|
1 x 1.5 ml |
3 x 1.5 ml |
5x Phusion™ GC Buffer |
|
1 x 1.5 ml |
1 x 1.5 ml |
dNTP mix |
10 mM/each |
100 µl |
400 µl |
MgCl2 solution |
50 mM |
1.5 ml |
1.5 ml |
Control lambda template |
0.5 ng/µl |
40 µl |
40 µl |
1.3 kb primers |
4 µM each |
50 µl |
50 µl |
10 kb primers |
4 µM each |
50 µl |
50 µl |
DNA size standard
(F-303SD) |
|
200 µl |
400 µl |
DMSO |
|
0.5 ml |
0.5 ml |
Ordering information
Phusion™ High-Fidelity PCR Kit |
Size |
F-553S |
50 reactions in 50 µl volume or 125 reactions in 20 µl volume |
F-553L |
200 reactions in 50 µl volume or 500 reactions in 20 µl volume |
|
Piko & Slidetiter demonstration movie high resolution (15.2MB)
Phusion™ High-Fidelity PCR Kit