Amplifying in the presence of raw blood. Blood, a well documented inhibitor of Taq-based PCR, completely blocked amplification of a 0.7 kb fragment from endogenous genomic DNA. To the contrary, High Performance PCR overcame the inhibitory effects of even high concentrations of blood and provided abundant yields of specific product. The High Performance PCR protocol time was under 30 minutes--less than half of that of conventional PCR. Positive control reactions contained purified genomic DNA in the absence of
whole blood.