The irrigating buffers and salt solutions are sterile physiological balanced solutions intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. These buffers and solutions are not cell culture media. They are composed of a phosphate buffer system, sodium chloride to adjust the osmolarity and perhaps sugar and metal ions for a short time for nutrition and stabilisation of morphology. For applications where Ca2+ and Mg2+ ions interfere with enzyme activity e.g. Trypsin, use the modified HBSS products.

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