Western Blot Imaging System, FluorChem Q
Summary
FluorChem Q System
Quantitative Western Blot and Fluorescent Imaging
The multiplex fluorescent detection and chemiluminescent imaging capabilities of the FluorChem Q provide a complete solution for quantitative Western blot imaging and analysis. The open platform is compatible with a wide variety of dyes and kits, providing the flexibility needed for multiple applications.
- Uniquely designed for quantitiative imaging of multiplex fluorescent westerns, with dyes such as Cy3 Cy5 and Cy2
- Fast lens technology also provides the ability to rapidly detect and quantitate chemiluminescent westerns without the need for film and a scanner
- Protocol driven analysis reducing imaging time by remembering the settings for imaging your assay
- Advanced AlphaView Q software provides the tools for analyzing your data, and provides the ability to save your analysis protocols for your assay
- Applications: Chemiluminescent Westerns, Multiplex Fluorescent Westerns, 96-Well Plates, 2-D Gels, Colony Counting, Protein Gels, DNA
Multiplex Western Analysis
Examine Multiple Proteins Simultaneously
The FluorChem Q allows quantitative detection of multiple proteins in a single assay, eliminating the need to strip and re-probe blots. Intensity values are easily normalized to a loading control to correct for inaccuracies.
Detect Co-Migrating Proteins
Overlapping proteins such as phosphorylated and unphosphorylated isoforms or other proteins with similar migration can be probed in the same blot. Our unique Channel Viewer feature allows visualization of individual bands in overlapping areas.
Ultra-Sensitive Protein Detection
The FluorChem Q System delivers the wide dynamic range and sensitivity essential to accurately detect subtle changes in protein levels.
Features And Benefits
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Six-position filter wheel provides UV and fluorescent imaging versatility - Three excitation channels with specific wavelengths for multiplex fluorescent Western blot imaging
- Powerful fixed lens for rapid imaging speeds
- AlphaView® Q software saves imaging protocols and provides quantitative analysis
Specifications
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Description |
Alphaimager FluorChem Q |
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Dimensions external HxWxD, mm |
100 x 50 x 38 cm |
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Field of View (Fast Lense) |
15 x 15 cm |
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Field of View (motorized lens) |
26 x 23 cm |
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Capacity |
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Weight, kg |
45kgs |
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Wavelength |
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Scientific grade Camera |
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Pixel size |
7.4 Micron Square |
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A/D Convertor |
16 bit |
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Resolution |
4.19 Megapixels |
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Absolutely Regulated |
-25Degrees for consistent imaging over long exposures and variable ambient. |
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Light Sources |
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UV Transilluminator |
302/365 nm Dual Wavelength UV Transilluminator (42 cm x 31 cm) |
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Epi White Light |
Dual Epi White Light Modules |
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Trans White Light |
Fold Down White Light Table(standard) |
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Epi UV |
254 nm, 365 nm or Dual 254/365 nm (Optional) |
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Cabinet |
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Filter Position |
Automated 6-position |
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Construction |
1pieces light tight casting. Upgradeable to Xplor for large format imaging. |
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Optics |
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Manual |
Manual fixed 50 mm f/0.95 Fast Lense optimized for Chemi, Standard in FCQ (Motorized lense optional) |
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Applications |
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Flourescence |
Yes |
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Chemiluminescence |
Yes |
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Absorbance/Colorimetric |
Yes |
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96 Well Plates |
Yes |
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Colony Plates |
Yes |
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Filters |
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Orange Filter (590nm, 50nm Band Pass) |
Ethidium Bromide and SYPRO Orange |
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Cy2 Filter (537nm, 35nm Band Pass) |
Cy2, DyLight 488, Alexa Flour 488 |
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Cy3 Filter (606nm, 68nm Band Pass) |
Cy3, DyLight 549, Alexa Flour 546/555 |
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Cy5 Filter (699nm, 62nm Band Pass) |
Cy5, DyLight 633/649/680, Alexa Flour 633/649/680 |
Ordering Information
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Part No |
Description |
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92-14116-00 |
FluorChem Q System includes a 4.19 MP camera with 16-bit A/D, cooled to -25°C absolute and regulated, 50 mm f/0.95 fixed lens, orange, Cy2, Cy3 and Cy5 filters, white light table, sample tray, 365/302 nm dual-wavelength, dual-intensity uv transilluminator, epi white lights, three specific epi excitation channels and alphaview Q system software with two stand alone licenses. |
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60-14329-00 |
FluorChem Xplor Upgrade for FluorChem Q extends FluorChem Q capability to include large format 1D (24 x 24 cm) and 2D protein gel imaging. includes f/1.8 manual fixed optics, red filter, XplorBright™ , Xploruv™ , sample tray, Xplor software and Xpedition™ 2D analysis software. |
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91-14507-00 |
Motorized Lens Replacement Option (for new FluorChem Q System orders only) replaces 50 mm, f/0.95 manual fixed lens with 50 mm, f/0.95 motorized fixed lens. |
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10-14794-00 |
Computer Comes pre-installed with alphaview System Software and is configured for optimal imaging system performance. |
